cell line a Search Results


a-172  (ATCC)
99
ATCC a-172
A 172, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a-172 - by Bioz Stars, 2026-03
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human lung carcinoma cell line a-549  (Chinou Jouhou Shisutemu)
90
Chinou Jouhou Shisutemu human lung carcinoma cell line a-549
Human Lung Carcinoma Cell Line A 549, supplied by Chinou Jouhou Shisutemu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human lung carcinoma cell line a-549/product/Chinou Jouhou Shisutemu
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human lung carcinoma cell line a-549 - by Bioz Stars, 2026-03
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submandibular gland squamous cell carcinoma cell line a-253  (National Research Council Canada)
90
National Research Council Canada submandibular gland squamous cell carcinoma cell line a-253
Submandibular Gland Squamous Cell Carcinoma Cell Line A 253, supplied by National Research Council Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/submandibular gland squamous cell carcinoma cell line a-253/product/National Research Council Canada
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submandibular gland squamous cell carcinoma cell line a-253 - by Bioz Stars, 2026-03
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brain cancer cell line a 127  (Pasteur Institute)
90
Pasteur Institute brain cancer cell line a 127
Brain Cancer Cell Line A 127, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain cancer cell line a 127 - by Bioz Stars, 2026-03
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gbm cell line a 172  (JCRB Cell Bank)
90
JCRB Cell Bank gbm cell line a 172
Suppression of cancer cell proliferation by AMF at 227 kHz for more than 30 min. (A) The effect of different frequencies (kHz) of AMF (250 Amrs) on the proliferation of GB cell lines (U87 and LN229). XTT cell proliferation assays were conducted at various AMF frequencies (kHz) for 30 min, with evaluation occurring 24 h post‐AMF exposure ( n = 4, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 kHz). (B) The impact of varying electric current intensities (Arms) in AMF (227 kHz) on the proliferation of GBM cell lines (U87 and LN229) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 Arms). (C) The effect of different exposure durations (min) to AMF (227 kHz, 250 Amrs) on the proliferation of GBM cell lines (LN229, <t>U251)</t> ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 min). (D) The influence of AMF (227 kHz, 250 Amrs) on other GB cell lines (U251, T98, and A172), a pancreatic cell line (PANC1), human breast cancer cell lines (MCF7, MDA‐MB‐231, MDA‐MB‐453), normal human astrocyte (NHA), human cardiac fibroblast (HCF), and human umbilical vein endothelial cells (HUVEC) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL). (E, F) Continuous monitoring of cell growth with and without a 30‐min AMF exposure (227 kHz, 250 Arms) in U251 and LN229 cell lines. In vitro cell proliferation was measured using the xCELLigence Real‐Time Cellular Analysis system. (G) Cell cycle analysis 3 and 24 h post‐AMF exposure (227 kHz, 250 Arms, 30 min), revealing the inhibitory effect of AMF, notably the induction of S and G2 phase arrest ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL). (H) Immunoblot analysis of phosphorylated and unphosphorylated forms of p53, p21, CDK2, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E, and GAPDH 24 h after a 30‐min AMF exposure (227 kHz, 250 Arms) ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL).
Gbm Cell Line A 172, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gbm cell line a 172/product/JCRB Cell Bank
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hatf3pro/gluc stable cell line a fosmid  (SpectraGenetics Inc)
90
SpectraGenetics Inc hatf3pro/gluc stable cell line a fosmid
Suppression of cancer cell proliferation by AMF at 227 kHz for more than 30 min. (A) The effect of different frequencies (kHz) of AMF (250 Amrs) on the proliferation of GB cell lines (U87 and LN229). XTT cell proliferation assays were conducted at various AMF frequencies (kHz) for 30 min, with evaluation occurring 24 h post‐AMF exposure ( n = 4, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 kHz). (B) The impact of varying electric current intensities (Arms) in AMF (227 kHz) on the proliferation of GBM cell lines (U87 and LN229) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 Arms). (C) The effect of different exposure durations (min) to AMF (227 kHz, 250 Amrs) on the proliferation of GBM cell lines (LN229, <t>U251)</t> ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 min). (D) The influence of AMF (227 kHz, 250 Amrs) on other GB cell lines (U251, T98, and A172), a pancreatic cell line (PANC1), human breast cancer cell lines (MCF7, MDA‐MB‐231, MDA‐MB‐453), normal human astrocyte (NHA), human cardiac fibroblast (HCF), and human umbilical vein endothelial cells (HUVEC) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL). (E, F) Continuous monitoring of cell growth with and without a 30‐min AMF exposure (227 kHz, 250 Arms) in U251 and LN229 cell lines. In vitro cell proliferation was measured using the xCELLigence Real‐Time Cellular Analysis system. (G) Cell cycle analysis 3 and 24 h post‐AMF exposure (227 kHz, 250 Arms, 30 min), revealing the inhibitory effect of AMF, notably the induction of S and G2 phase arrest ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL). (H) Immunoblot analysis of phosphorylated and unphosphorylated forms of p53, p21, CDK2, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E, and GAPDH 24 h after a 30‐min AMF exposure (227 kHz, 250 Arms) ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL).
Hatf3pro/Gluc Stable Cell Line A Fosmid, supplied by SpectraGenetics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hatf3pro/gluc stable cell line a fosmid/product/SpectraGenetics Inc
Average 90 stars, based on 1 article reviews
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a-375  (ATCC)
99
ATCC a-375
Suppression of cancer cell proliferation by AMF at 227 kHz for more than 30 min. (A) The effect of different frequencies (kHz) of AMF (250 Amrs) on the proliferation of GB cell lines (U87 and LN229). XTT cell proliferation assays were conducted at various AMF frequencies (kHz) for 30 min, with evaluation occurring 24 h post‐AMF exposure ( n = 4, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 kHz). (B) The impact of varying electric current intensities (Arms) in AMF (227 kHz) on the proliferation of GBM cell lines (U87 and LN229) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 Arms). (C) The effect of different exposure durations (min) to AMF (227 kHz, 250 Amrs) on the proliferation of GBM cell lines (LN229, <t>U251)</t> ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 min). (D) The influence of AMF (227 kHz, 250 Amrs) on other GB cell lines (U251, T98, and A172), a pancreatic cell line (PANC1), human breast cancer cell lines (MCF7, MDA‐MB‐231, MDA‐MB‐453), normal human astrocyte (NHA), human cardiac fibroblast (HCF), and human umbilical vein endothelial cells (HUVEC) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL). (E, F) Continuous monitoring of cell growth with and without a 30‐min AMF exposure (227 kHz, 250 Arms) in U251 and LN229 cell lines. In vitro cell proliferation was measured using the xCELLigence Real‐Time Cellular Analysis system. (G) Cell cycle analysis 3 and 24 h post‐AMF exposure (227 kHz, 250 Arms, 30 min), revealing the inhibitory effect of AMF, notably the induction of S and G2 phase arrest ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL). (H) Immunoblot analysis of phosphorylated and unphosphorylated forms of p53, p21, CDK2, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E, and GAPDH 24 h after a 30‐min AMF exposure (227 kHz, 250 Arms) ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL).
A 375, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a-375/product/ATCC
Average 99 stars, based on 1 article reviews
a-375 - by Bioz Stars, 2026-03
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human kidney cell line a-293  (Genentech inc)
90
Genentech inc human kidney cell line a-293
Suppression of cancer cell proliferation by AMF at 227 kHz for more than 30 min. (A) The effect of different frequencies (kHz) of AMF (250 Amrs) on the proliferation of GB cell lines (U87 and LN229). XTT cell proliferation assays were conducted at various AMF frequencies (kHz) for 30 min, with evaluation occurring 24 h post‐AMF exposure ( n = 4, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 kHz). (B) The impact of varying electric current intensities (Arms) in AMF (227 kHz) on the proliferation of GBM cell lines (U87 and LN229) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 Arms). (C) The effect of different exposure durations (min) to AMF (227 kHz, 250 Amrs) on the proliferation of GBM cell lines (LN229, <t>U251)</t> ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 min). (D) The influence of AMF (227 kHz, 250 Amrs) on other GB cell lines (U251, T98, and A172), a pancreatic cell line (PANC1), human breast cancer cell lines (MCF7, MDA‐MB‐231, MDA‐MB‐453), normal human astrocyte (NHA), human cardiac fibroblast (HCF), and human umbilical vein endothelial cells (HUVEC) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL). (E, F) Continuous monitoring of cell growth with and without a 30‐min AMF exposure (227 kHz, 250 Arms) in U251 and LN229 cell lines. In vitro cell proliferation was measured using the xCELLigence Real‐Time Cellular Analysis system. (G) Cell cycle analysis 3 and 24 h post‐AMF exposure (227 kHz, 250 Arms, 30 min), revealing the inhibitory effect of AMF, notably the induction of S and G2 phase arrest ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL). (H) Immunoblot analysis of phosphorylated and unphosphorylated forms of p53, p21, CDK2, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E, and GAPDH 24 h after a 30‐min AMF exposure (227 kHz, 250 Arms) ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL).
Human Kidney Cell Line A 293, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human kidney cell line a-293/product/Genentech inc
Average 90 stars, based on 1 article reviews
human kidney cell line a-293 - by Bioz Stars, 2026-03
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marc-145 cell line, a stable and highly expressing porcine cd163 (marc-145cd163)  (Biowit Technologies)
90
Biowit Technologies marc-145 cell line, a stable and highly expressing porcine cd163 (marc-145cd163)
Suppression of cancer cell proliferation by AMF at 227 kHz for more than 30 min. (A) The effect of different frequencies (kHz) of AMF (250 Amrs) on the proliferation of GB cell lines (U87 and LN229). XTT cell proliferation assays were conducted at various AMF frequencies (kHz) for 30 min, with evaluation occurring 24 h post‐AMF exposure ( n = 4, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 kHz). (B) The impact of varying electric current intensities (Arms) in AMF (227 kHz) on the proliferation of GBM cell lines (U87 and LN229) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 Arms). (C) The effect of different exposure durations (min) to AMF (227 kHz, 250 Amrs) on the proliferation of GBM cell lines (LN229, <t>U251)</t> ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 min). (D) The influence of AMF (227 kHz, 250 Amrs) on other GB cell lines (U251, T98, and A172), a pancreatic cell line (PANC1), human breast cancer cell lines (MCF7, MDA‐MB‐231, MDA‐MB‐453), normal human astrocyte (NHA), human cardiac fibroblast (HCF), and human umbilical vein endothelial cells (HUVEC) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL). (E, F) Continuous monitoring of cell growth with and without a 30‐min AMF exposure (227 kHz, 250 Arms) in U251 and LN229 cell lines. In vitro cell proliferation was measured using the xCELLigence Real‐Time Cellular Analysis system. (G) Cell cycle analysis 3 and 24 h post‐AMF exposure (227 kHz, 250 Arms, 30 min), revealing the inhibitory effect of AMF, notably the induction of S and G2 phase arrest ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL). (H) Immunoblot analysis of phosphorylated and unphosphorylated forms of p53, p21, CDK2, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E, and GAPDH 24 h after a 30‐min AMF exposure (227 kHz, 250 Arms) ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL).
Marc 145 Cell Line, A Stable And Highly Expressing Porcine Cd163 (Marc 145cd163), supplied by Biowit Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/marc-145 cell line, a stable and highly expressing porcine cd163 (marc-145cd163)/product/Biowit Technologies
Average 90 stars, based on 1 article reviews
marc-145 cell line, a stable and highly expressing porcine cd163 (marc-145cd163) - by Bioz Stars, 2026-03
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proprietary cell line a producing an mab m20301  (ABL Bio)
90
ABL Bio proprietary cell line a producing an mab m20301
Suppression of cancer cell proliferation by AMF at 227 kHz for more than 30 min. (A) The effect of different frequencies (kHz) of AMF (250 Amrs) on the proliferation of GB cell lines (U87 and LN229). XTT cell proliferation assays were conducted at various AMF frequencies (kHz) for 30 min, with evaluation occurring 24 h post‐AMF exposure ( n = 4, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 kHz). (B) The impact of varying electric current intensities (Arms) in AMF (227 kHz) on the proliferation of GBM cell lines (U87 and LN229) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 Arms). (C) The effect of different exposure durations (min) to AMF (227 kHz, 250 Amrs) on the proliferation of GBM cell lines (LN229, <t>U251)</t> ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 min). (D) The influence of AMF (227 kHz, 250 Amrs) on other GB cell lines (U251, T98, and A172), a pancreatic cell line (PANC1), human breast cancer cell lines (MCF7, MDA‐MB‐231, MDA‐MB‐453), normal human astrocyte (NHA), human cardiac fibroblast (HCF), and human umbilical vein endothelial cells (HUVEC) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL). (E, F) Continuous monitoring of cell growth with and without a 30‐min AMF exposure (227 kHz, 250 Arms) in U251 and LN229 cell lines. In vitro cell proliferation was measured using the xCELLigence Real‐Time Cellular Analysis system. (G) Cell cycle analysis 3 and 24 h post‐AMF exposure (227 kHz, 250 Arms, 30 min), revealing the inhibitory effect of AMF, notably the induction of S and G2 phase arrest ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL). (H) Immunoblot analysis of phosphorylated and unphosphorylated forms of p53, p21, CDK2, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E, and GAPDH 24 h after a 30‐min AMF exposure (227 kHz, 250 Arms) ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL).
Proprietary Cell Line A Producing An Mab M20301, supplied by ABL Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proprietary cell line a producing an mab m20301/product/ABL Bio
Average 90 stars, based on 1 article reviews
proprietary cell line a producing an mab m20301 - by Bioz Stars, 2026-03
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cell line a-549  (Gibthai Co Ltd)
90
Gibthai Co Ltd cell line a-549
Suppression of cancer cell proliferation by AMF at 227 kHz for more than 30 min. (A) The effect of different frequencies (kHz) of AMF (250 Amrs) on the proliferation of GB cell lines (U87 and LN229). XTT cell proliferation assays were conducted at various AMF frequencies (kHz) for 30 min, with evaluation occurring 24 h post‐AMF exposure ( n = 4, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 kHz). (B) The impact of varying electric current intensities (Arms) in AMF (227 kHz) on the proliferation of GBM cell lines (U87 and LN229) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 Arms). (C) The effect of different exposure durations (min) to AMF (227 kHz, 250 Amrs) on the proliferation of GBM cell lines (LN229, <t>U251)</t> ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 min). (D) The influence of AMF (227 kHz, 250 Amrs) on other GB cell lines (U251, T98, and A172), a pancreatic cell line (PANC1), human breast cancer cell lines (MCF7, MDA‐MB‐231, MDA‐MB‐453), normal human astrocyte (NHA), human cardiac fibroblast (HCF), and human umbilical vein endothelial cells (HUVEC) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL). (E, F) Continuous monitoring of cell growth with and without a 30‐min AMF exposure (227 kHz, 250 Arms) in U251 and LN229 cell lines. In vitro cell proliferation was measured using the xCELLigence Real‐Time Cellular Analysis system. (G) Cell cycle analysis 3 and 24 h post‐AMF exposure (227 kHz, 250 Arms, 30 min), revealing the inhibitory effect of AMF, notably the induction of S and G2 phase arrest ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL). (H) Immunoblot analysis of phosphorylated and unphosphorylated forms of p53, p21, CDK2, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E, and GAPDH 24 h after a 30‐min AMF exposure (227 kHz, 250 Arms) ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL).
Cell Line A 549, supplied by Gibthai Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cell line a-549 - by Bioz Stars, 2026-03
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tsh-responsive cell line a cho cell line  (Interthyr Corporation)
90
Interthyr Corporation tsh-responsive cell line a cho cell line
Suppression of cancer cell proliferation by AMF at 227 kHz for more than 30 min. (A) The effect of different frequencies (kHz) of AMF (250 Amrs) on the proliferation of GB cell lines (U87 and LN229). XTT cell proliferation assays were conducted at various AMF frequencies (kHz) for 30 min, with evaluation occurring 24 h post‐AMF exposure ( n = 4, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 kHz). (B) The impact of varying electric current intensities (Arms) in AMF (227 kHz) on the proliferation of GBM cell lines (U87 and LN229) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 Arms). (C) The effect of different exposure durations (min) to AMF (227 kHz, 250 Amrs) on the proliferation of GBM cell lines (LN229, <t>U251)</t> ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 min). (D) The influence of AMF (227 kHz, 250 Amrs) on other GB cell lines (U251, T98, and A172), a pancreatic cell line (PANC1), human breast cancer cell lines (MCF7, MDA‐MB‐231, MDA‐MB‐453), normal human astrocyte (NHA), human cardiac fibroblast (HCF), and human umbilical vein endothelial cells (HUVEC) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL). (E, F) Continuous monitoring of cell growth with and without a 30‐min AMF exposure (227 kHz, 250 Arms) in U251 and LN229 cell lines. In vitro cell proliferation was measured using the xCELLigence Real‐Time Cellular Analysis system. (G) Cell cycle analysis 3 and 24 h post‐AMF exposure (227 kHz, 250 Arms, 30 min), revealing the inhibitory effect of AMF, notably the induction of S and G2 phase arrest ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL). (H) Immunoblot analysis of phosphorylated and unphosphorylated forms of p53, p21, CDK2, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E, and GAPDH 24 h after a 30‐min AMF exposure (227 kHz, 250 Arms) ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL).
Tsh Responsive Cell Line A Cho Cell Line, supplied by Interthyr Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tsh-responsive cell line a cho cell line - by Bioz Stars, 2026-03
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Image Search Results


Suppression of cancer cell proliferation by AMF at 227 kHz for more than 30 min. (A) The effect of different frequencies (kHz) of AMF (250 Amrs) on the proliferation of GB cell lines (U87 and LN229). XTT cell proliferation assays were conducted at various AMF frequencies (kHz) for 30 min, with evaluation occurring 24 h post‐AMF exposure ( n = 4, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 kHz). (B) The impact of varying electric current intensities (Arms) in AMF (227 kHz) on the proliferation of GBM cell lines (U87 and LN229) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 Arms). (C) The effect of different exposure durations (min) to AMF (227 kHz, 250 Amrs) on the proliferation of GBM cell lines (LN229, U251) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 min). (D) The influence of AMF (227 kHz, 250 Amrs) on other GB cell lines (U251, T98, and A172), a pancreatic cell line (PANC1), human breast cancer cell lines (MCF7, MDA‐MB‐231, MDA‐MB‐453), normal human astrocyte (NHA), human cardiac fibroblast (HCF), and human umbilical vein endothelial cells (HUVEC) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL). (E, F) Continuous monitoring of cell growth with and without a 30‐min AMF exposure (227 kHz, 250 Arms) in U251 and LN229 cell lines. In vitro cell proliferation was measured using the xCELLigence Real‐Time Cellular Analysis system. (G) Cell cycle analysis 3 and 24 h post‐AMF exposure (227 kHz, 250 Arms, 30 min), revealing the inhibitory effect of AMF, notably the induction of S and G2 phase arrest ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL). (H) Immunoblot analysis of phosphorylated and unphosphorylated forms of p53, p21, CDK2, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E, and GAPDH 24 h after a 30‐min AMF exposure (227 kHz, 250 Arms) ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL).

Journal: Cancer Science

Article Title: Alternative magnetic field exposure suppresses tumor growth via metabolic reprogramming

doi: 10.1111/cas.16243

Figure Lengend Snippet: Suppression of cancer cell proliferation by AMF at 227 kHz for more than 30 min. (A) The effect of different frequencies (kHz) of AMF (250 Amrs) on the proliferation of GB cell lines (U87 and LN229). XTT cell proliferation assays were conducted at various AMF frequencies (kHz) for 30 min, with evaluation occurring 24 h post‐AMF exposure ( n = 4, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 kHz). (B) The impact of varying electric current intensities (Arms) in AMF (227 kHz) on the proliferation of GBM cell lines (U87 and LN229) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 Arms). (C) The effect of different exposure durations (min) to AMF (227 kHz, 250 Amrs) on the proliferation of GBM cell lines (LN229, U251) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 min). (D) The influence of AMF (227 kHz, 250 Amrs) on other GB cell lines (U251, T98, and A172), a pancreatic cell line (PANC1), human breast cancer cell lines (MCF7, MDA‐MB‐231, MDA‐MB‐453), normal human astrocyte (NHA), human cardiac fibroblast (HCF), and human umbilical vein endothelial cells (HUVEC) ( n = 4, ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL). (E, F) Continuous monitoring of cell growth with and without a 30‐min AMF exposure (227 kHz, 250 Arms) in U251 and LN229 cell lines. In vitro cell proliferation was measured using the xCELLigence Real‐Time Cellular Analysis system. (G) Cell cycle analysis 3 and 24 h post‐AMF exposure (227 kHz, 250 Arms, 30 min), revealing the inhibitory effect of AMF, notably the induction of S and G2 phase arrest ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL). (H) Immunoblot analysis of phosphorylated and unphosphorylated forms of p53, p21, CDK2, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E, and GAPDH 24 h after a 30‐min AMF exposure (227 kHz, 250 Arms) ( n = 4, ns, not significant, ** p < 0.01, *** p < 0.001 vs. CTRL).

Article Snippet: Human GBM cell lines, U251 MG‐Luc (U251, JCRB1386) and A‐172 (A172, JCRB0228) were purchased from the Japanese Collection of Research Bioresources (JCRB) Cell Bank.

Techniques: In Vitro, Cell Cycle Assay, Western Blot